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Precision run-on sequencing (PRO-seq) for microbiome transcriptomics - Nature Microbiology

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Precision run-on sequencing (PRO-seq) for microbiome transcriptomics - Nature Microbiology

Bacteria respond to environmental stimuli through precise regulation of transcription initiation and elongation. Bulk RNA sequencing primarily characterizes mature transcripts, so to identify actively transcribed loci we need to capture RNA polymerase (RNAP) complexed with nascent RNA. However, such capture methods have only previously been applied to culturable, genetically tractable organisms such as E. coli and B. subtilis.

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Precision run-on sequencing (PRO-seq) for microbiome transcriptomics - Nature Microbiology

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Precision run-on sequencing (PRO-seq) for microbiome transcriptomics - Nature Microbiology

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