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Short-range translocation by a restriction enzyme motor triggers diffusion along DNA - Nature Chemical Biology

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Short-range translocation by a restriction enzyme motor triggers diffusion along DNA - Nature Chemical Biology

Cleavage of bacteriophage DNA by the Type III restriction-modification enzymes requires long-range interaction between DNA sites. This is facilitated by one-dimensional diffusion ('DNA sliding') initiated by ATP hydrolysis catalyzed by a superfamily 2 helicase-like ATPase. Here we combined ultrafast twist measurements based on plasmonic DNA origami nano-rotors with stopped-flow fluorescence and gel-based assays to examine the role(s) of ATP hydrolysis.

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Short-range translocation by a restriction enzyme motor triggers diffusion along DNA - Nature Chemical Biology

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Short-range translocation by a restriction enzyme motor triggers diffusion along DNA - Nature Chemical Biology

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