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The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo - Nature Methods

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The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo - Nature Methods

Multiphoton microscopy can resolve fluorescent structures and dynamics deep in scattering tissue and has transformed neural imaging, but applying this technique in vivo can be limited by the mechanical and optical constraints of conventional objectives. Short working distance objectives can collide with compact surgical windows or other instrumentation and preclude imaging. Here we present an ultra-long working distance (20 mm) air objective called the Cousa objective.

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The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo - Nature Methods

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The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo - Nature Methods

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