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Design of a redox-proficient Escherichia coli for screening terpenoids and modifying cytochrome P450s - Nature Catalysis

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Design of a redox-proficient Escherichia coli for screening terpenoids and modifying cytochrome P450s - Nature Catalysis

High-value terpenoids are found in plants, animals and microbes, with applications spanning health to agriculture. However, moving their biosynthetic pathways to a new host is challenging when cytochrome P450 (CYP) enzymes are needed for function. Here we engineer Escherichia coli to facilitate discovery by introducing 31 recombinant genes that enhance precursor supply, combine electron transfer pathways and implement regulatory control.

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Design of a redox-proficient Escherichia coli for screening terpenoids and modifying cytochrome P450s - Nature Catalysis

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Design of a redox-proficient Escherichia coli for screening terpenoids and modifying cytochrome P450s - Nature Catalysis

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