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Intrinsic RNA-targeting activity limits the applicability of CRISPR–Cas13 - Nature Biomedical Engineering
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We revealed that the RNA-targeting activity of the Cas13 family of nucleases allows them to directly target endogenous RNA in mammalian cells. Such activity limits the usage of lentiviral Cas13 systems, and suggests a need for caution when applying Cas13-based systems.
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