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Minimally invasive longitudinal intravital imaging of cellular dynamics in intact long bone - Nature Protocols

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Minimally invasive longitudinal intravital imaging of cellular dynamics in intact long bone - Nature Protocols

Intravital two-photon microscopy enables deep-tissue imaging at high temporospatial resolution in live animals. However, the endosteal bone compartment and underlying bone marrow pose unique challenges to optical imaging as light is absorbed, scattered and dispersed by thick mineralized bone matrix and the adipose-rich bone marrow. Early bone intravital imaging methods exploited gaps in the cranial sutures to bypass the need to penetrate through cortical bone.

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Minimally invasive longitudinal intravital imaging of cellular dynamics in intact long bone - Nature Protocols

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Minimally invasive longitudinal intravital imaging of cellular dynamics in intact long bone - Nature Protocols

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