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A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome - Nature Structural & Molecular Biology

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A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome - Nature Structural & Molecular Biology

RNA modifications are widespread in biology and abundant in ribosomal RNA. However, the importance of these modifications is not well understood. We show that methylation of a single nucleotide, in the catalytic center of the large subunit, gates ribosome assembly. Massively parallel mutational scanning of the essential nuclear GTPase Nog2 identified important interactions with rRNA, particularly with the 2′-O-methylated A-site base Gm2922.

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A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome - Nature Structural & Molecular Biology

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A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome - Nature Structural & Molecular Biology

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