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27 days ago

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Masked acid chlorides for proximity labelling of RNA - Nature Chemistry

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Masked acid chlorides for proximity labelling of RNA - Nature Chemistry

A non-radical proximity labelling platform — BAP-seq — is presented that uses subcellular-localized BS2 esterase to convert unreactive enol-based probes into highly reactive acid chlorides in situ to label nearby RNAs. When paired with click-handle-mediated enrichment and sequencing, this chemistry enables high-resolution spatial mapping of RNAs across subcellular compartments.

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Masked acid chlorides for proximity labelling of RNA - Nature Chemistry

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Masked acid chlorides for proximity labelling of RNA - Nature Chemistry

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