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Selective-plane-activation structured illumination microscopy - Nature Methods

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Selective-plane-activation structured illumination microscopy - Nature Methods

The background light from out-of-focus planes hinders resolution enhancement in structured illumination microscopy when observing volumetric samples. Here we used selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background.

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Selective-plane-activation structured illumination microscopy - Nature Methods

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Selective-plane-activation structured illumination microscopy - Nature Methods

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