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Engineering APOBEC3A deaminase for highly accurate and efficient base editing - Nature Chemical Biology

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Engineering APOBEC3A deaminase for highly accurate and efficient base editing - Nature Chemical Biology

Cytosine base editors (CBEs) are effective tools for introducing C-to-T base conversions, but their clinical applications are limited by off-target and bystander effects. Through structure-guided engineering of human APOBEC3A (A3A) deaminase, we developed highly accurate A3A-CBE (haA3A-CBE) variants that efficiently generate C-to-T conversion with a narrow editing window and near-background level of DNA and RNA off-target activity, irrespective of methylation status and sequence context.

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Engineering APOBEC3A deaminase for highly accurate and efficient base editing - Nature Chemical Biology

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Engineering APOBEC3A deaminase for highly accurate and efficient base editing - Nature Chemical Biology

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