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A universal system for boosting gene expression in eukaryotic cell-lines - Nature Communications

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A universal system for boosting gene expression in eukaryotic cell-lines - Nature Communications

We demonstrate a transcriptional regulatory design algorithm that can boost expression in yeast and mammalian cell lines. The system consists of a simplified transcriptional architecture composed of a minimal core promoter and a synthetic upstream regulatory region (sURS) composed of up to three motifs selected from a list of 41 motifs conserved in the eukaryotic lineage. The sURS system was first characterized using an oligo-library containing 189,990 variants.

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A universal system for boosting gene expression in eukaryotic cell-lines - Nature Communications

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A universal system for boosting gene expression in eukaryotic cell-lines - Nature Communications

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