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An optogenetic method for the controlled release of single molecules - Nature Methods

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An optogenetic method for the controlled release of single molecules - Nature Methods

We developed a system for optogenetic release of single molecules in cells. We confined soluble and transmembrane proteins to the Golgi apparatus via a photocleavable protein and released them by short pulses of light. Our method allows for a light dose-dependent delivery of functional proteins to the cytosol and plasma membrane in amounts compatible with single-molecule imaging, greatly simplifying access to single-molecule microscopy of any protein in live cells.

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An optogenetic method for the controlled release of single molecules - Nature Methods

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An optogenetic method for the controlled release of single molecules - Nature Methods

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