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Cost and time-efficient construction of a 3′-end mRNA library from unpurified bulk RNA in a single tube - Experimental & Molecular Medicine

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Cost and time-efficient construction of a 3′-end mRNA library from unpurified bulk RNA in a single tube - Experimental & Molecular Medicine

The major drawbacks of RNA sequencing (RNA-seq), a remarkably accurate transcriptome profiling method, is its high cost and poor scalability. Here, we report a highly scalable and cost-effective method for transcriptomics profiling called Bulk transcriptOme profiling of cell Lysate in a single poT (BOLT-seq), which is performed using unpurified bulk 3′-end mRNA in crude cell lysates.

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Cost and time-efficient construction of a 3′-end mRNA library from unpurified bulk RNA in a single tube - Experimental & Molecular Medicine

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Cost and time-efficient construction of a 3′-end mRNA library from unpurified bulk RNA in a single tube - Experimental & Molecular Medicine

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Unlocking the Power of Knowledge
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