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Super-sectioning with multi-sheet reversible saturable optical fluorescence transitions (RESOLFT) microscopy - Nature Methods

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Super-sectioning with multi-sheet reversible saturable optical fluorescence transitions (RESOLFT) microscopy - Nature Methods

Light-sheet fluorescence microscopy is an invaluable tool for four-dimensional biological imaging of multicellular systems due to the rapid volumetric imaging and minimal illumination dosage. However, it is challenging to retrieve fine subcellular information, especially in living cells, due to the width of the sheet of light (>1 μm).

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Super-sectioning with multi-sheet reversible saturable optical fluorescence transitions (RESOLFT) microscopy - Nature Methods

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Super-sectioning with multi-sheet reversible saturable optical fluorescence transitions (RESOLFT) microscopy - Nature Methods

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