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Harnessing eukaryotic retroelement proteins for transgene insertion into human safe-harbor loci - Nature Biotechnology

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Harnessing eukaryotic retroelement proteins for transgene insertion into human safe-harbor loci - Nature Biotechnology

Current approaches for inserting autonomous transgenes into the genome, such as CRISPR–Cas9 or virus-based strategies, have limitations including low efficiency and high risk of untargeted genome mutagenesis. Here, we describe precise RNA-mediated insertion of transgenes (PRINT), an approach for site-specifically primed reverse transcription that directs transgene synthesis directly into the genome at a multicopy safe-harbor locus.

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Harnessing eukaryotic retroelement proteins for transgene insertion into human safe-harbor loci - Nature Biotechnology

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Harnessing eukaryotic retroelement proteins for transgene insertion into human safe-harbor loci - Nature Biotechnology

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