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Immobilized enzyme cascade for targeted glycosylation - Nature Chemical Biology

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Immobilized enzyme cascade for targeted glycosylation - Nature Chemical Biology

Glycosylation is a critical post-translational protein modification that affects folding, half-life and functionality. Glycosylation is a non-templated and heterogeneous process because of the promiscuity of the enzymes involved. We describe a platform for sequential glycosylation reactions for tailored sugar structures (SUGAR-TARGET) that allows bespoke, controlled N-linked glycosylation in vitro enabled by immobilized enzymes produced with a one-step immobilization/purification method.

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Immobilized enzyme cascade for targeted glycosylation - Nature Chemical Biology

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Immobilized enzyme cascade for targeted glycosylation - Nature Chemical Biology

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