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Homotrimer barcodes enable accurate counting of RNA molecules during high-throughput RNA sequencing - Nature Methods

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Homotrimer barcodes enable accurate counting of RNA molecules during high-throughput RNA sequencing - Nature Methods

We pinpoint PCR artifacts as the primary source of inaccurate quantification in both short- and long-read RNA sequencing, a problem that intensifies with an increase in PCR cycles in both bulk and single-cell sequencing contexts. To overcome this challenge, we engineered a novel unique molecular identifier (UMI) barcode composed of homotrimer nucleotide blocks. This design facilitates accurate quantification of RNA molecules, substantially improving molecular counting.

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Homotrimer barcodes enable accurate counting of RNA molecules during high-throughput RNA sequencing - Nature Methods

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Homotrimer barcodes enable accurate counting of RNA molecules during high-throughput RNA sequencing - Nature Methods

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