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Correcting PCR amplification errors in unique molecular identifiers to generate accurate numbers of sequencing molecules - Nature Methods

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Correcting PCR amplification errors in unique molecular identifiers to generate accurate numbers of sequencing molecules - Nature Methods

Unique molecular identifiers are random oligonucleotide sequences that remove PCR amplification biases. However, the impact that PCR associated sequencing errors have on the accuracy of generating absolute counts of RNA molecules is underappreciated.

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Correcting PCR amplification errors in unique molecular identifiers to generate accurate numbers of sequencing molecules - Nature Methods

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Correcting PCR amplification errors in unique molecular identifiers to generate accurate numbers of sequencing molecules - Nature Methods

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