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In vitro reconstitution of chromatin domains shows a role for nucleosome positioning in 3D genome organization - Nature Genetics

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In vitro reconstitution of chromatin domains shows a role for nucleosome positioning in 3D genome organization - Nature Genetics

Eukaryotic genomes are organized into chromatin domains. The molecular mechanisms driving the formation of these domains are difficult to dissect in vivo and remain poorly understood. Here we reconstitute Saccharomyces cerevisiae chromatin in vitro and determine its 3D organization at subnucleosome resolution by micrococcal nuclease-based chromosome conformation capture and molecular dynamics simulations.

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In vitro reconstitution of chromatin domains shows a role for nucleosome positioning in 3D genome organization - Nature Genetics

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In vitro reconstitution of chromatin domains shows a role for nucleosome positioning in 3D genome organization - Nature Genetics

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