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Fluorogenic CRISPR for genomic DNA imaging - Nature Communications

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Fluorogenic CRISPR for genomic DNA imaging - Nature Communications

Genomic DNA exhibits high heterogeneity in terms of its dynamic within the nucleus, its structure and functional roles. CRISPR-based imaging approaches can image genomic loci in living cells. However, conventional CRISPR-based tools involve expressing constitutively fluorescent proteins, resulting in high background and nonspecific nucleolar signal. Here, we construct fluorogenic CRISPR (fCRISPR) to overcome these issues.

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Fluorogenic CRISPR for genomic DNA imaging - Nature Communications

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Fluorogenic CRISPR for genomic DNA imaging - Nature Communications

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