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Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome - Nature Communications

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Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome - Nature Communications

Abundant cellular transcripts occupy most of the sequencing reads in the transcriptome, making it challenging to assay for low-abundant transcripts. Here, we utilize the adaptive sampling function of Oxford Nanopore sequencing to selectively deplete and enrich RNAs of interest without biochemical manipulation before sequencing. Adaptive sampling performed on a pool of in vitro transcribed RNAs resulted in a net increase of 22-30% in the proportion of transcripts of interest in the population.

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Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome - Nature Communications

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Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome - Nature Communications

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