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Irreversible light-activated SpyLigation mediates split-protein assembly in 4D - Nature Protocols

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Irreversible light-activated SpyLigation mediates split-protein assembly in 4D - Nature Protocols

The conditional assembly of split-protein pairs to modulate biological activity is commonly achieved by fusing split-protein fragments to dimerizing components that bring inactive pairs into close proximity in response to an exogenous trigger. However, current methods lack full spatial and temporal control over reconstitution, require sustained activation and lack specificity.

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Irreversible light-activated SpyLigation mediates split-protein assembly in 4D - Nature Protocols

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Irreversible light-activated SpyLigation mediates split-protein assembly in 4D - Nature Protocols

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