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Mirror-image trypsin digestion and sequencing of D-proteins - Nature Chemistry

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Mirror-image trypsin digestion and sequencing of D-proteins - Nature Chemistry

The development of mirror-image biology systems and related applications is hindered by the lack of effective methods to sequence mirror-image (D-) proteins. Although natural-chirality (L-) proteins can be sequenced by bottom–up liquid chromatography–tandem mass spectrometry (LC–MS/MS), the sequencing of long D-peptides and D-proteins with the same strategy requires digestion by a site-specific D-protease before mass analysis.

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Mirror-image trypsin digestion and sequencing of D-proteins - Nature Chemistry

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