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Massively parallel single-cell sequencing of diverse microbial populations - Nature Methods

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Massively parallel single-cell sequencing of diverse microbial populations - Nature Methods

Single-cell genetic heterogeneity is ubiquitous in microbial populations and an important aspect of microbial biology; however, we lack a broadly applicable and accessible method to study this heterogeneity in microbial populations. Here, we show a simple, robust and generalizable method for high-throughput single-cell sequencing of target genetic loci in diverse microbes using simple droplet microfluidics devices (droplet targeted amplicon sequencing; DoTA-seq).

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Massively parallel single-cell sequencing of diverse microbial populations - Nature Methods

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Massively parallel single-cell sequencing of diverse microbial populations - Nature Methods

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