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Profiling human brain vascular cells using single-cell transcriptomics and organoids - Nature Protocols

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Profiling human brain vascular cells using single-cell transcriptomics and organoids - Nature Protocols

Angiogenesis and neurogenesis are functionally interconnected during brain development. However, the study of the vasculature has trailed other brain cell types because they are delicate and of low abundance. Here we describe a protocol extension to purify prenatal human brain endothelial and mural cells with FACS and utilize them in downstream applications, including transcriptomics, culture and organoid transplantation.

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Profiling human brain vascular cells using single-cell transcriptomics and organoids - Nature Protocols

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Profiling human brain vascular cells using single-cell transcriptomics and organoids - Nature Protocols

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