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Generation of programmable splicing factors using RNA-binding proteins that activate exon inclusion - Nature Biotechnology

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Generation of programmable splicing factors using RNA-binding proteins that activate exon inclusion - Nature Biotechnology

We developed luciferase-based splicing reporters to evaluate 718 RNA-binding proteins (RBPs) for the ability to activate exon inclusion. Our screen detected RBPs with no previously characterized roles in splicing and utilized RBP domains displaying potent exon activation to develop programmable splicing modulators with improved strength, generalizability and size over previous versions.

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Generation of programmable splicing factors using RNA-binding proteins that activate exon inclusion - Nature Biotechnology

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Generation of programmable splicing factors using RNA-binding proteins that activate exon inclusion - Nature Biotechnology

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