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Complete set of the Atg8–E1–E2–E3 conjugation machinery forms an interaction web that mediates membrane shaping - Nature Structural & Molecular Biology

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Complete set of the Atg8–E1–E2–E3 conjugation machinery forms an interaction web that mediates membrane shaping - Nature Structural & Molecular Biology

Atg8, a ubiquitin-like protein, is conjugated with phosphatidylethanolamine (PE) via Atg7 (E1), Atg3 (E2) and Atg12–Atg5–Atg16 (E3) enzymatic cascade and mediates autophagy. However, its molecular roles in autophagosome formation are still unclear. Here we show that Saccharomyces cerevisiae Atg8–PE and E1–E2–E3 enzymes together construct a stable, mobile membrane scaffold.

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Complete set of the Atg8–E1–E2–E3 conjugation machinery forms an interaction web that mediates membrane shaping - Nature Structural & Molecular Biology

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Complete set of the Atg8–E1–E2–E3 conjugation machinery forms an interaction web that mediates membrane shaping - Nature Structural & Molecular Biology

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