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A nanobody-based strategy for rapid and scalable purification of human protein complexes - Nature Protocols

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A nanobody-based strategy for rapid and scalable purification of human protein complexes - Nature Protocols

The isolation of proteins in high yield and purity is a major bottleneck for the analysis of their three-dimensional structure, function and interactome. Here, we present a streamlined workflow for the rapid production of proteins or protein complexes using lentiviral transduction of human suspension cells, combined with highly specific nanobody-mediated purification and proteolytic elution.

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A nanobody-based strategy for rapid and scalable purification of human protein complexes - Nature Protocols

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A nanobody-based strategy for rapid and scalable purification of human protein complexes - Nature Protocols

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