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Genetically encoded multimeric tags for subcellular protein localization in cryo-EM - Nature Methods

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Genetically encoded multimeric tags for subcellular protein localization in cryo-EM - Nature Methods

Cryo-electron tomography (cryo-ET) allows for label-free high-resolution imaging of macromolecular assemblies in their native cellular context. However, the localization of macromolecules of interest in tomographic volumes can be challenging. Here we present a ligand-inducible labeling strategy for intracellular proteins based on fluorescent, 25-nm-sized, genetically encoded multimeric particles (GEMs).

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Genetically encoded multimeric tags for subcellular protein localization in cryo-EM - Nature Methods

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Genetically encoded multimeric tags for subcellular protein localization in cryo-EM - Nature Methods

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