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An antioxidant screen identifies ascorbic acid for prevention of light-induced mitotic prolongation in live cell imaging - Communications Biology

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An antioxidant screen identifies ascorbic acid for prevention of light-induced mitotic prolongation in live cell imaging - Communications Biology

Phototoxicity is an important issue in fluorescence live imaging of light-sensitive cellular processes such as mitosis. Among several approaches to reduce phototoxicity, the addition of antioxidants to the media has been used as a simple method. Here, we analyzed the impact of phototoxicity on the mitotic progression in fluorescence live imaging of human cells and performed a screen to identify the most efficient antioxidative agents that reduce it.

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An antioxidant screen identifies ascorbic acid for prevention of light-induced mitotic prolongation in live cell imaging - Communications Biology

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An antioxidant screen identifies ascorbic acid for prevention of light-induced mitotic prolongation in live cell imaging - Communications Biology

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