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Assembly of complete mouse embryo models from embryonic and induced stem cell types in vitro - Nature Protocols

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Assembly of complete mouse embryo models from embryonic and induced stem cell types in vitro - Nature Protocols

The interaction between embryonic and extraembryonic tissues is critical in natural mouse embryogenesis. Here, to enable such interaction in vitro, we describe a protocol to assemble a complete mouse embryo model using mouse embryonic stem cells and induced embryonic stem cells to express Cdx2 (or trophoblast stem cells) and Gata4 to reconstitute the epiblast, extraembryonic ectoderm and visceral endoderm lineages, respectively.

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Assembly of complete mouse embryo models from embryonic and induced stem cell types in vitro - Nature Protocols

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Assembly of complete mouse embryo models from embryonic and induced stem cell types in vitro - Nature Protocols

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