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Assigning functionality to cysteines by base editing of cancer dependency genes - Nature Chemical Biology

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Assigning functionality to cysteines by base editing of cancer dependency genes - Nature Chemical Biology

Covalent chemistry represents an attractive strategy for expanding the ligandability of the proteome, and chemical proteomics has revealed numerous electrophile-reactive cysteines on diverse human proteins. Determining which of these covalent binding events affect protein function, however, remains challenging. Here we describe a base-editing strategy to infer the functionality of cysteines by quantifying the impact of their missense mutation on cancer cell proliferation.

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Assigning functionality to cysteines by base editing of cancer dependency genes - Nature Chemical Biology

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Assigning functionality to cysteines by base editing of cancer dependency genes - Nature Chemical Biology

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