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Transcriptional linkage analysis with in vivo AAV-Perturb-seq - Nature

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Transcriptional linkage analysis with in vivo AAV-Perturb-seq - Nature

The ever-growing compendium of genetic variants associated with human pathologies demands new methods to study genotype–phenotype relationships in complex tissues in a high-throughput manner1,2. Here we introduce adeno-associated virus (AAV)-mediated direct in vivo single-cell CRISPR screening, termed AAV-Perturb-seq, a tuneable and broadly applicable method for transcriptional linkage analysis as well as high-throughput and high-resolution phenotyping of genetic perturbations in vivo.

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Transcriptional linkage analysis with in vivo AAV-Perturb-seq - Nature

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Transcriptional linkage analysis with in vivo AAV-Perturb-seq - Nature

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