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Genotoxic effects of base and prime editing in human hematopoietic stem cells - Nature Biotechnology

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Genotoxic effects of base and prime editing in human hematopoietic stem cells - Nature Biotechnology

Base and prime editors (BEs and PEs) may provide more precise genetic engineering than nuclease-based approaches because they bypass the dependence on DNA double-strand breaks. However, little is known about their cellular responses and genotoxicity. Here, we compared state-of-the-art BEs and PEs and Cas9 in human hematopoietic stem and progenitor cells with respect to editing efficiency, cytotoxicity, transcriptomic changes and on-target and genome-wide genotoxicity.

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Genotoxic effects of base and prime editing in human hematopoietic stem cells - Nature Biotechnology

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Genotoxic effects of base and prime editing in human hematopoietic stem cells - Nature Biotechnology

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