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Enhanced active-site electric field accelerates enzyme catalysis - Nature Chemistry

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Enhanced active-site electric field accelerates enzyme catalysis - Nature Chemistry

The design and improvement of enzymes based on physical principles remain challenging. Here we demonstrate that the principle of electrostatic catalysis can be leveraged to substantially improve a natural enzyme's activity. We enhanced the active-site electric field in horse liver alcohol dehydrogenase by replacing the serine hydrogen-bond donor with threonine and replacing the catalytic Zn2+ with Co2+.

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Enhanced active-site electric field accelerates enzyme catalysis - Nature Chemistry

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Enhanced active-site electric field accelerates enzyme catalysis - Nature Chemistry

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