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Large-scale growth of C. elegans and isolation of membrane protein complexes - Nature Protocols

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Large-scale growth of C. elegans and isolation of membrane protein complexes - Nature Protocols

Purification of membrane proteins for biochemical and structural studies is commonly achieved by recombinant overexpression in heterologous cell lines. However, many membrane proteins do not form a functional complex in a heterologous system, and few methods exist to purify sufficient protein from a native source for use in biochemical, biophysical and structural studies. Here, we provide a detailed protocol for the isolation of membrane protein complexes from transgenic Caenorhabditis elegans.

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Large-scale growth of C. elegans and isolation of membrane protein complexes - Nature Protocols

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Large-scale growth of C. elegans and isolation of membrane protein complexes - Nature Protocols

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