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Engineered bacterial orthogonal DNA replication system for continuous evolution - Nature Chemical Biology

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Engineered bacterial orthogonal DNA replication system for continuous evolution - Nature Chemical Biology

Continuous evolution can generate biomolecules for synthetic biology and enable evolutionary investigation. The orthogonal DNA replication system (OrthoRep) in yeast can efficiently mutate long DNA fragments in an easy-to-operate manner. However, such a system is lacking in bacteria. Therefore, we developed a bacterial orthogonal DNA replication system (BacORep) for continuous evolution.

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Engineered bacterial orthogonal DNA replication system for continuous evolution - Nature Chemical Biology

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Engineered bacterial orthogonal DNA replication system for continuous evolution - Nature Chemical Biology

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