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Single-cell quantification of ribosome occupancy in early mouse development - Nature

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Single-cell quantification of ribosome occupancy in early mouse development - Nature

Translation regulation is critical for early mammalian embryonic development1. However, previous studies had been restricted to bulk measurements2, precluding precise determination of translation regulation including allele-specific analyses. Here, to address this challenge, we developed a novel microfluidic isotachophoresis (ITP) approach, named RIBOsome profiling via ITP (Ribo-ITP), and characterized translation in single oocytes and embryos during early mouse development.

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Single-cell quantification of ribosome occupancy in early mouse development - Nature

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Single-cell quantification of ribosome occupancy in early mouse development - Nature

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