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Photo-expansion microscopy enables super-resolution imaging of cells embedded in 3D hydrogels - Nature Materials

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Photo-expansion microscopy enables super-resolution imaging of cells embedded in 3D hydrogels - Nature Materials

Hydrogels are extensively used as tunable, biomimetic three-dimensional cell culture matrices, but optically deep, high-resolution images are often difficult to obtain, limiting nanoscale quantification of cell–matrix interactions and outside-in signalling. Here we present photopolymerized hydrogels for expansion microscopy that enable optical clearance and tunable ×4.6–6.7 homogeneous expansion of not only monolayer cell cultures and tissue sections, but cells embedded within hydrogels.

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Photo-expansion microscopy enables super-resolution imaging of cells embedded in 3D hydrogels - Nature Materials

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Photo-expansion microscopy enables super-resolution imaging of cells embedded in 3D hydrogels - Nature Materials

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