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A highly sensitive and robust one-pot assay for the detection of microRNAs - Nature Biomedical Engineering

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A highly sensitive and robust one-pot assay for the detection of microRNAs - Nature Biomedical Engineering

We developed exponentially amplified rolling circle amplification with CRISPR–Cas12a as a one-pot, isothermal assay for microRNA detection. This method has single-digit femtomolar sensitivity and single-nucleotide specificity, and can be deployed for point-of-care testing. The assay has been adapted for the microRNA profiling of extracellular vesicles, which is used in the diagnosis of pancreatic cancer.

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A highly sensitive and robust one-pot assay for the detection of microRNAs - Nature Biomedical Engineering

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A highly sensitive and robust one-pot assay for the detection of microRNAs - Nature Biomedical Engineering

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