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Large-scale F0 CRISPR screens in vivo using MIC-Drop - Nature Protocols

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Large-scale F0 CRISPR screens in vivo using MIC-Drop - Nature Protocols

The zebrafish is a powerful model system for studying animal development, for modeling genetic diseases, and for large-scale in vivo functional genetics. Because of its ease of use and its high efficiency in targeted gene perturbation, CRISPR–Cas9 has recently gained prominence as the tool of choice for genetic manipulation in zebrafish. However, scaling up the technique for high-throughput in vivo functional genetics has been a challenge.

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Large-scale F0 CRISPR screens in vivo using MIC-Drop - Nature Protocols

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Large-scale F0 CRISPR screens in vivo using MIC-Drop - Nature Protocols

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