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Time-resolved assessment of single-cell protein secretion by sequencing - Nature Methods

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Time-resolved assessment of single-cell protein secretion by sequencing - Nature Methods

Secreted proteins play critical roles in cellular communication. Methods enabling concurrent measurement of cellular protein secretion, phenotypes and transcriptomes are still unavailable. Here we describe time-resolved assessment of protein secretion from single cells by sequencing (TRAPS-seq). Released proteins are trapped onto the cell surface and probed by oligonucleotide-barcoded antibodies before being simultaneously sequenced with transcriptomes in single cells.

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Time-resolved assessment of single-cell protein secretion by sequencing - Nature Methods

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Time-resolved assessment of single-cell protein secretion by sequencing - Nature Methods

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