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Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+ - Nature Methods

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Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+ - Nature Methods

Discovery of off-target CRISPR–Cas activity in patient-derived cells and animal models is crucial for genome editing applications, but currently exhibits low sensitivity. We demonstrate that inhibition of DNA-dependent protein kinase catalytic subunit accumulates the repair protein MRE11 at CRISPR–Cas-targeted sites, enabling high-sensitivity mapping of off-target sites to positions of MRE11 binding using chromatin immunoprecipitation followed by sequencing.

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Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+ - Nature Methods

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Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+ - Nature Methods

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