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TnpB structure reveals minimal functional core of Cas12 nuclease family - Nature

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TnpB structure reveals minimal functional core of Cas12 nuclease family - Nature

The widespread TnpB proteins of IS200/IS605 transposon family have recently emerged as the smallest RNA-guided nucleases capable of targeted genome editing in eukaryotic cells1,2. Bioinformatic analysis identified TnpB proteins as the likely predecessors of Cas12 nucleases3–5, which along with Cas9 are widely used for targeted genome manipulation. Whereas Cas12 family nucleases are well characterized both biochemically and structurally6, the molecular mechanism of TnpB remains unknown.

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TnpB structure reveals minimal functional core of Cas12 nuclease family - Nature

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TnpB structure reveals minimal functional core of Cas12 nuclease family - Nature

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