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Quantitative profiling of pseudouridylation landscape in the human transcriptome - Nature Chemical Biology

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Quantitative profiling of pseudouridylation landscape in the human transcriptome - Nature Chemical Biology

Pseudouridine (Ψ) is an abundant post-transcriptional RNA modification in ncRNA and mRNA. However, stoichiometric measurement of individual Ψ sites in human transcriptome remains unaddressed. Here we develop 'PRAISE', via selective chemical labeling of Ψ by bisulfite to induce nucleotide deletion signature during reverse transcription, to realize quantitative assessment of the Ψ landscape in the human transcriptome.

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quantitative transcriptome chemical biology chemical landscape pseudouridylation biology health

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Quantitative profiling of pseudouridylation landscape in the human transcriptome - Nature Chemical Biology

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Quantitative profiling of pseudouridylation landscape in the human transcriptome - Nature Chemical Biology

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