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Targeted DNA integration in human cells without double-strand breaks using CRISPR-associated transposases - Nature Biotechnology

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Targeted DNA integration in human cells without double-strand breaks using CRISPR-associated transposases - Nature Biotechnology

Conventional genome engineering with CRISPR–Cas9 creates double-strand breaks (DSBs) that lead to undesirable byproducts and reduce product purity. Here we report an approach for programmable integration of large DNA sequences in human cells that avoids the generation of DSBs by using Type I-F CRISPR-associated transposases (CASTs).

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Targeted DNA integration in human cells without double-strand breaks using CRISPR-associated transposases - Nature Biotechnology

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Targeted DNA integration in human cells without double-strand breaks using CRISPR-associated transposases - Nature Biotechnology

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