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Global detection of human variants and isoforms by deep proteome sequencing - Nature Biotechnology

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Global detection of human variants and isoforms by deep proteome sequencing - Nature Biotechnology

An average shotgun proteomics experiment detects approximately 10,000 human proteins from a single sample. However, individual proteins are typically identified by peptide sequences representing a small fraction of their total amino acids. Hence, an average shotgun experiment fails to distinguish different protein variants and isoforms. Deeper proteome sequencing is therefore required for the global discovery of protein isoforms.

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Global detection of human variants and isoforms by deep proteome sequencing - Nature Biotechnology

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Global detection of human variants and isoforms by deep proteome sequencing - Nature Biotechnology

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