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CRISPR-Csm for eukaryotic RNA knockdown and imaging without toxicity - Nature Biotechnology

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CRISPR-Csm for eukaryotic RNA knockdown and imaging without toxicity - Nature Biotechnology

Targeted RNA degradation remains a challenge using existing methods owing to off-target effects and toxicity. We adapted the CRISPR-Csm complex, a multi-protein effector from type III CRISPR systems, for precise knockdown of nuclear or cytoplasmic transcripts in eukaryotic cells with minimal side effects.

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CRISPR-Csm for eukaryotic RNA knockdown and imaging without toxicity - Nature Biotechnology

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CRISPR-Csm for eukaryotic RNA knockdown and imaging without toxicity - Nature Biotechnology

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