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In vitro reconstitution of COPII vesicles from Arabidopsis thaliana suspension-cultured cells - Nature Protocols

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In vitro reconstitution of COPII vesicles from Arabidopsis thaliana suspension-cultured cells - Nature Protocols

Transport vesicles mediate protein traffic between endomembrane organelles in a highly selective and efficient manner. In vitro reconstitution systems have been widely used for studying mechanisms of vesicle formation, polar trafficking, and cargo specificity in mammals and yeast. However, this technique has not yet been applied to plants because of the large lytic vacuoles and rigid cell walls.

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In vitro reconstitution of COPII vesicles from Arabidopsis thaliana suspension-cultured cells - Nature Protocols

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In vitro reconstitution of COPII vesicles from Arabidopsis thaliana suspension-cultured cells - Nature Protocols

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