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Accurate isoform discovery with IsoQuant using long reads - Nature Biotechnology

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Accurate isoform discovery with IsoQuant using long reads - Nature Biotechnology

Annotating newly sequenced genomes and determining alternative isoforms from long-read RNA data are complex and incompletely solved problems. Here we present IsoQuant—a computational tool using intron graphs that accurately reconstructs transcripts both with and without reference genome annotation. For novel transcript discovery, IsoQuant reduces the false-positive rate fivefold and 2.5-fold for Oxford Nanopore reference-based or reference-free mode, respectively.

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Accurate isoform discovery with IsoQuant using long reads - Nature Biotechnology

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Accurate isoform discovery with IsoQuant using long reads - Nature Biotechnology

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